IVB Conclusions

Cost and Productivity Goals The overall conclusion from this review of 2 decades of DOE and ASP R& D in microalgal mass culture for biodiesel and other renewable fuels, is that this technology still requires relatively long-term R& D for practical realization. The initial, rather optimistic, cost and performance projections have not been met, or when met Th. pexfmnn exp stateree co fo rop rossuc tiy(ety)hiavveedlgeen era.is ed This in ft gyeaS) aetqlODyhp liodiuowt g dactoor...

Le Algal Biomass Production

This project addressed the problem of the uncontrolled nature of the algal populations in wastewater treatment ponds. Because the dominant algal species found in a pond could range from small unicellular to large colonial or filamentous species, harvesting of the algae for biomass conversion would require a universally applicable harvesting technology, such as centrifugation or chemical flocculation, to enable the recovery of any algal type. However, these processes are very expensive (Golueke...

Laboratory Studies

The objectives of this subcontract were to collect microalgal strains from a variety of locations iC the'desert regions' of an to screen them for their ability to grow under conditions in a commercial microalgal biodiesel facility. Studies were also conducted to optimize a fluorometric procedure for CstBecttiig caps p co entwi toApii thi9 85etllll d Ju gregl8a .mew attehg samps containing microalgae were collected from 125 sites in Arizona, California, Nevada, New Mexico, Texas, and Utah. Some...

Iiid Nrel Studies of Flue Gas CO Utilization by Microalgae

The last major engineering-related activity carried out by the ASP was a PETC-funded study, both laboratory and process design, for microalgae biodiesel production using power plant flue gases. The general arguments for use of microalgae in CO2 mitigation, from high productivity to relatively low water use were reviewed in several reports Brown et al. 1991 Chelf et al. 1991 . The authors concluded that the SOx and NOx impurities in flue gas would likely not have a major effect on algal...

IIIB The Israeli Microalgae Biodiesel Production Project

In the mid-1980s an algal mass culture project for biodiesel production was supported by the ASP in Israel Arad 1984, 1985, 1986 , as a cooperative project amo1ng the M eMnce gnogpsphic and Limnological Research Institute, with Dr. Ben-Amotz, who investigated lipid production at the laboratory and micropond scale 2 Ben-Gurion University of the Negev, with Professor A. Richmond as Principal Investigator, investigating algal mass cultures with outdoor ponds, essentially of the HRP design. 3...

SERIInHouse Researchers

Tornabene, T.G. 1983 Chemical profile of algae with emphasis on lipids of microalgae. Aquatic Species Program Review Proceedings of the March 1983 Principal Investigators' Meeting, Solar Energy Research Institute, Golden, -6Gp homaSPW .H. 1985 Chemical profiles of selected species of microalgae with emphasis on lipids. J. Phycol. 21 72-81. Tornabene, T.G. Holzer, G. Peterson, S.L. 1980 Lipid profile of the halophilic alga, Dunaliella salina. Biochem. Biophys. Res. Comm. 96...

IIIA An Integrated System for the Conversion of Solar Energy withSewage Grown Microalgae

Wastewater Treatment Using Algae

During FY1976-1977, the project described in Section III.A.2. continued with the same overall objective to determine what pond operating factors could allow control over algal species, and thus permit cultivation of algal types that allow low-cost harvesting by microstrainers. The biomass recycling process described earlier continued to be studied, using the 12-m rectangular ponds and various pond AherllbteIfsS0eaae igi 0fxirprtspnga s rWgtghtidDliliimesahi lnllaa smcarc 1gp Chdaiowith dgsy...

Fertilizer

This 2-year project Benemann et al. 1979 Eisenberg et al. 1980 extended from the end of 1977 to Winter 1979. Initially the approach to establish microstrainable cultures using the 12-m ponds, described in Section III.A.3., continued to be investigated. Essentially the same results as before were obtained detention time was found to be the key environmental variable determining algal colony size but not necessarily species composition and a negative correlation was found between numbers of algal...

Info

Western blot, showing the presence of the 30-kDa NPTII protein in transformed cells of C. cryptica T13L and N. saprophila. In this example, C. cryptica and N. saprophila were transformed with pACCNPT10 and pACCNPT5.1, respectively. Crude cell extracts were separated on SDS-polyacrylamide gels, blotted onto a nitrocellulose filter, and NPTII protein was detected using anti-NPTII primary antibodies and alkaline phosphatase-conjugated goat anti-rabbit IgG secondary antibodies. The...

Collection Screening and Characterization of Microalgae

Collection, Screening, and Characterization of Microalgae by SERIIn-House Researchers SERI researchers also started to evaluate various strains by the rapid screening procedure. Initial work focused on 25 partially characterized strains. These strains were analyzed for growth and Nile Red fluorescence in exponentially growing cultures and in cultures grown under N-deficient conditions for 4 days. The results of the SERI and Sommerfeld laboratories cannot be compared directly, because Nile Red...