rapidly to ensure consistent results. The kinetics of fluorescence in stained cells varied in different species, presumably due to differences in the permeability of cell walls to the stain, and differences in how the lipid is stored in the cells, i.e., as large or small droplets. Fixing the stained cells with formaldehyde or ethanol preserved the Nile Red fluorescence for 2 hours, but cells that were chemically fixed before Nile Red staining did not exhibit the characteristic yellow fluorescence. When Nile Red fluorescence was measured in algal cultures over time, the fluorescence increased as
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